In vitro vegetative growth and flowering of olive tree in response to GA3 treatment

The phytohormone gibberellin is involved in the regulation of many physiological process including flower induction and shoot growth. In this study, gibberellic acid (GA3) was used in order to induce thereversion of olive tree vegetative buds towards a floral ones in vitro. For this, six varieties (Marsaline, Chemchali, Picholine, Chemlali, Zalmati and Oueslati ) was tested and explants, consisting of a singlenode segments, were grown in media containing three concentrations of GA3 (1, 2 and 10 mg/l). Results show that Marsaline seems to be the most able variety in regenerating floral structures. For this variety,5 cases of reversion were observed mainly on the medium containing 10 mg/l GA3. This same medium was also favorable for this transformation for the other varieties (3 cases on Picholine, one each on Chemchali , Zalmati and Oueslati). The examination of the histological sections confirmed this transformation. In addition, this experiment showed that GA3 can be at the origin of an interestinggrowth rate of vegetative buds, which elongation depend on variety as well as GA3 concentration

A collision-induced satellite in the Lyman Beta profile due to H-H collisions

We present a theoretical profile of the Lyman Beta line of atomic hydrogen perturbed by collisions with neutral hydrogen atoms and protons. We use a general unified theory in which the electric dipole moment varies during a collision. A collision-induced satellite appears on Lyman Beta, correlated to the B''\barB 1Sigma+u - X 1Sigma+g asymptotically forbidden transition of H_2. As a consequence, the appearance of the line wing between Lyman Alpha and Lyman Beta is shown to be sensitive to the relative abundance of hydrogen ions and neutral atoms, and thereby to provide a temperature diagnostic for stellar atmospheres and laboratory plasmas.Comment: 8 pages, 4 figures, acc.f.publ.European Physical Journal

Heating effects on some quality caracteristics of date seed oil.

peer reviewe

Investigation of genetic variability related to the in vitro floral hermaphrodism induction in Date palm (Phoenix dactylifera L.)

This paper reports on a molecular analysis study conducted on Date palm flowers from the Deglet Nour cultivar to investigate putative genetic variability related to the in vitro floral hermaphrodism induction. Natural male and female as well as hermaphrodite ones that were produced in vitro through the hormonal treatment of female flowers were submitted to ISSR-PCR analysis. Microsatellite based amplification (ISSR) was applied on genomic DNA from inflorescences taken at different periods of hormonal treatment corresponding to the various deviation stages to search for putative variations that may have occurred on the initial genome due to the application of plant growth regulators. Several amplification bands were purified, cloned, and sequenced. The results revealed that hormonal treatment entailed no detectable genetic variation in the treated Date palm flowers. Two of the selected and ISSR-PCR amplified DNA fragments showed however, possible links with flowering regulation. The findings indicate that these sequences are potential candidate gene markers that may enhance our understanding of flower development and sex identification in this species.Key words: Date palm, female inflorescences, hermaphrodite flowers, in vitro culture, ISSR, sex identification

Quasi-molecular lines in Lyman wings of cool DA white dwarfs; Application to FUSE observations of G231-40

We present new theoretical calculations of the total line profiles of Lyman alpha and Lyman beta which include perturbations by both neutral hydrogen AND protons and all possible quasi-molecular states of H_2 and H_2^+. They are used to improve theoretical modeling of synthetic spectra for cool DA white dwarfs. We compare them with FUSE observation of G231-40. The appearance of the line wings between Lyman alpha and Lyman beta is shown to be sensitive to the relative abundance of hydrogen ions and neutral atoms, and thereby to provide a temperature diagnostic for stellar atmospheres and laboratory plasmas.Comment: 6 pages, 4 figures, accepted for publication in Astronomy and Astrophysic

In vitro cryopreservation of date palm caulogenic meristems

Cryopreservation is the technology of choice not only for plant genetic resource preservation but also for virus eradication and for the efficient management of large-scale micropropagation. In this chapter, we describe three cryopreservation protocols (standard vitrification, droplet vitrification, and encapsulation vitrification) for date palm highly proliferating meristems that are initiated from vitro-cultures using plant growth regulator-free MS medium. The positive impact of sucrose preculture and cold hardening treatments on survival rates is significant. Regeneration rates obtained with standard vitrification, encapsulation-vitrification, and droplet-vitrification protocols can reach 30, 40, and 70%, respectively. All regenerated plants from non-cryopreserved or cryopreserved explants don't show morphological variation by maintaining genetic integrity without adverse effect of cryogenic treatment. Cryopreservation of date palm vitro-cultures enables commercial tissue culture laboratories to move to large-scale propagation from cryopreserved cell lines producing true-to-type plants after clonal field-testing trials. When comparing the cost of cryostorage and in-field conservation of date palm cultivars, tissue cryopreservation is the most cost-effective. Moreover, many of the risks linked to field conservation like erosion due to climatic, edaphic, and phytopathologic constraints are circumvented. (Résumé d'auteur

Enhanced Graph Rewriting Systems for Complex Software Domain

International audienceMethodologies for correct by construction reconfigurations can efficiently solve consistency issues in dynamic software architecture. Graph-based models are appropriate for designing such architectures and methods. At the same time, they may be unfit to characterize a system from a non functional perspective. This stems from efficiency and applicability limitations in handling time-varying characteristics and their related dependencies. In order to lift these restrictions, an extension to graph rewriting systems is proposed herein. The suitability of this approach, as well as the restraints of currently available ones, are illustrated, analysed and experimentally evaluated with reference to a concrete example. This investigation demonstrates that the conceived solution can: (i) express any kind of algebraic dependencies between evolving requirements and properties; (ii) significantly ameliorate the efficiency and scalability of system modifications with respect to classic methodologies; (iii) provide an efficient access to attribute values; (iv) be fruitfully exploited in software management systems; (v) guarantee theoretical properties of a grammar, like its termination

Genetic diversity at the Dhn3 locus in Turkish Hordeum spontaneum populations with comparative structural analyses

We analysed Hordeum spontaneum accessions from 21 different locations to understand the genetic diversity of HsDhn3 alleles and effects of single base mutations on the intrinsically disordered structure of the resulting polypeptide (HsDHN3). HsDHN3 was found to be YSK2-type with a low-frequency 6-aa deletion in the beginning of Exon 1. There is relatively high diversity in the intron region of HsDhn3 compared to the two exon regions. We have found subtle differences in K segments led to changes in amino acids chemical properties. Predictions for protein interaction profiles suggest the presence of a protein-binding site in HsDHN3 that coincides with the K1 segment. Comparison of DHN3 to closely related cereals showed that all of them contain a nuclear localization signal sequence flanking to the K1 segment and a novel conserved region located between the S and K1 segments [E(D/T)DGMGGR]. We found that H. vulgare, H. spontaneum, and Triticum urartu DHN3s have a greater number of phosphorylation sites for protein kinase C than other cereal species, which may be related to stress adaptation. Our results show that the nature and extent of mutations in the conserved segments of K1 and K2 are likely to be key factors in protection of cells